The skin is considered the largest organ of the body and has many different functions. The skin functions in thermoregulation, protection, metabolic functions and sensation. One of the primary functions of the skin is protection. Protection is accomplished via a two-way barrier function whereby the loss of water to the external environment is reduced and the risk of contamination from external substances and organisms is decreased. Maintaining this barrier is essential for health of the patient. Damage to the skin can upset the biochemical balance and barrier capacity of skin. The purpose of this is to study investigate skin physiology and changes to its histological structure as result of such damage. Two skin care formulations, one containing trypsin (proteolytic enzyme) as an active ingredient and another containing dimethicone as an active ingredient were evaluated for their effects on the barrier function, elasticity and pH of the skin. Barrier function was evaluated via TEWL, elasticity via Corneometry and pH via Skin pH Meter. The data indicates that the trypsin may result in an increased rate of TEWL. Skin pH is somewhat effected. No changes in the elasticity were noted. The dimethicone had no apparent effects to the 3 parameters evaluated.