The WOCN Society 40th Annual Conference (June 21-25th, 2008)


2407

An in vitro Assessment of the Release Rate of Silver from a Unique Hydrocellular Foam Dressing Containing Antimicrobial Silver

David W. Brett, BS, BS, MS, Smith & Nephew, Science & Technology Manager, 11775 Starkey Rd, Largo, FL 33773

Wound Bed Preparation can be described as the management of the wound to accelerate endogenous healing or to facilitate the effectiveness of other therapeutic measures. Two key aspects of Wound Bed Preparation are controlling bioburden and moisture levels in the wound. The antimicrobial efficacy of ionic silver is well known as is the fluid handling capabilities of hydrocellular foams. The level of silver is key as the antimicrobial efficacy of silver is dose dependent. A therapeutic concentration of silver is necessary for rapid kill of a wide variety of pathogens and associated strains of those pathogens. In this study, the rate, level and time frame of silver release from the afore mentioned dressing is investigated. A weighed 2x2cm sample was placed into a sealable container, wound contact layer down. 5ml of horse serum was transferred to the sealable container, then the sample was pressed with a glass rod to ensure wetting out, before storing without shaking, at 37oC. After 24 hours, the horse serum was decanted into a new sealable container and the sample was squeezed to remove as much fluid from the container as possible, without disturbing any particulate matter present. The extracts were stored under refrigeration until the analysis was carried out. The procedure was repeated after 2, 3, 4, 5, 6 and 7 days, then all of the extracts were analyzed for silver content using Inductively Coupled Plasma -Optical Emission Spectroscopy (ICP-OES). The results indicate that a bactericidal level of silver (40 ppm - 80 ppm) is released over a period of 7 days with the maximum level of silver achieved within 4 hrs, then decreasing steadily over a 7 day period.