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Ceramide are lipids existing between epidermal corneocytes. The ceramide assumes water retention and a barrier function of the horny layer and it is widely known as a moisturizing ingredient. Pads containing of the ceramides have been recently in use for the purpose of skin protection. The amount of ceramide has been recently in use in the horny layer decreases with aging. The decrease of ceramide causes dry skin and the lowering of its barrier function, increasing susceptibility to the external stimulation. We examined the effects skin wound using a skin barrier of ceramide combined with hydrocolloid, on unique mouse model with a slight skin deficit.
Method
A wound healing delayed mouse (db/db) was given inhalation anesthesia with Isoflurane and slight skin deficit was created on it is to back using CO2 laser. The experimental group patched ceramide combined skin barrier to the wound, and the control group patched no ceramide combined skin barrier to the wound. We peeled off the skin barrier a week later and performed macroscopic observation and histological investigation. In addition, we compared the transepidermal water loss (TEWL) and between the groups to quantity the skin barrier effect of ceramide-combined patches.
Results
The group with patched ceramide-combined skin barrier showed significant macroscopic, and histologically promotion of epidermal cell reproduction. In addition, the TEWL measured lower in the experimental group than in the control group.
Discussion
Ceramide have been reported to possess water retention and barrier functions of the horny layer, the promotional effects as well as to promote increase of keratinocytes and epithelization when metabolized by glucosylceramide. This experiment suggests the ceramide combined skin barrier has on an epidermis reproduction, ceramide combined skin barrier were consistent with the results obtained in practical knowledge. We will inspect the mechanism of ceramide hastening healing of skin wound.