Wound Bed Preparation (WBP) can be described as the management of the wound to accelerate endogenous healing or to facilitate the effectiveness of other therapeutic measures. The 4 basic aspects of WBP can now be represented by an acronym, TIME T= tissue (non-viable or deficient), addressed clinically via debridement. I= infection or inflammation, addressed clinically via antimicrobial agents. M= moisture control, addressed clinically via absorbent dressings and/or compression. E= epidermal margin, addressed clinically via tissue engineered constructs/grafts. Focusing on the “I” in TIME, there are numerous antimicrobial dressings that are intended to fulfill this need. As wound dressings are introduced it becomes important to determine their antimicrobial activity and compare them with preexisting treatments. As there are numerous silver dressings available to the clinician, there is value in comparing the efficacy of other (non-silver) antimicrobial agents to them. The purpose of this study is to investigate the antimicrobial efficacy of a cadexomer iodine antimicrobial dressing as it relates to a variety of silver dressings. To assess antimicrobial activity all dressings were applied to non-woven absorbent material. Solutions of antibiotic resistant P. aeruginosa, E. coli, Streptococcus, methacyllin resistant S. aureus (MRSA) and vancomycin resistant E. faecium (VRE) were added to absorbent material (control) and antimicrobial dressings. The log reduction was calculated (in triplicate) on the control and test dressings. The in vitro analysis provides insight into the dressing functionality in the clinic.