Wound Bed Preparation (WBP) can be described as the management of the wound to accelerate endogenous healing or to facilitate the effectiveness of other therapeutic measures. The 4 basic aspects of WBP can now be represented by an acronym, TIME T= tissue (non-viable or deficient), addressed clinically via debridement. I= infection or inflammation, addressed clinically via antimicrobial agents. M= moisture control, addressed clinically via absorbent dressings and/or compression. E= epidermal margin, addressed clinically via tissue engineered constructs/grafts. Focusing on the “I” in TIME, there are numerous antimicrobial dressings that are intended to fulfill this need. As new and innovative wound care treatments are introduced into the market place, it is imperative to begin to investigate their compatibility with pre-existing treatments. Nanocrystalline silver-coated dressings have become popular antimicrobial barrier dressings use in wound care. In some clinical situations, a combined application of a nanocrystalline silver dressing with another type of wound care technology is required. Therefore, the impact of other wound care products on the antimicrobial activities of nanocrystalline silver dressings needs to be understood. The purpose of this study is to evaluate the antimicrobial activity by measurements of the in vitro reduction in viable bacterial counts and by the corrected zone of inhibition. Nineteen different dressing/combinations were evaluated in triplicate. The results show that by and large all products tested had no effect on the antimicrobial efficacy of the nanocrystalline silver-coated dressing with few exceptions. Only bacterial collagenase deactivated the nanocrystalline silver dressing.