Recent studies have demonstrated that the enzymatic debriding agent papain–urea copper chlorophyllin (PUC) can facilitate wound healing, but its effect on quality of healing is not well understood. The purpose of this study was to compare re-epithelialization and overall wound healing in acute, infected wounds treated with either PUC, its individual components used alone, or moist wound care alone. Twenty full-thickness wounds were created on the dorsum of pigs. Following hemostasis, the wounds were contaminated with wound-isolated bacteria and then dressed with either a moist dressing alone, or one of the test articles (PUC, papain-urea, copper chlorophyllin, or urea-based ointment) and a moisture-retentive dressing. Wounds were evaluated on days 1, 4, 8, 11, 14, 18, and 21 postsurgery. There was no difference in the rate of epithelialization in the different treatment groups. Microscopic examination, however, revealed greater depth of rete pegs and a greater number of keratinocytes in the epidermis of PUC and papain-treated wounds. Blood vessel formation was also higher in PUC, papain, and copper chlorophyllin-treated wounds than in those treated with moist dressings alone. In conclusion, the healing in PUC-treated wounds appeared to be more complete based on the number of keratinocytes present in the epidermis, the more extensive rete peg formation, and greater degree of vascularization. The effect on rete ridges and the number of keratinocytes in the epidermis appeared to be due to the papain-urea component of PUC, while all components appeared to contribute to the greater degree of vascularization.